The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface

摘要

The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.